Anti-Collagen I COL1A1 Monoclonal Antibody

Product Name

Anti-Collagen I COL1A1 Monoclonal Antibody

View all COL1A1 Antibodies

SKU/Catalog Number

M00329-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Collagen I COL1A1 Monoclonal Antibody catalog # M00329-1. Tested in IF, IHC-P, ICC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen I COL1A1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00329-1)

Host

Mouse

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

1F12

Isotype

IgG

Immunogen

synthetic peptide derived from Collagen I.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00329-1 is reactive to COL1A1 in Human, Mouse, Rat

Applications

M00329-1 is guaranteed for IF, IHC-P, ICC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

138.941kDa

Background of COL1A1

Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC-P 1:50-300
IF 1:50-200

Validation Images & Assay Conditions

Click image to see more details

Immunohistochemical analysis of paraffin-embedded human skin.
Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue.
Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded human skin.
Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Click image to see more details

Immunohistochemical analysis of paraffin-embedded human skin.
Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Click image to see more details

Immunohistochemical analysis of paraffin-embedded rat kidney tissue.
Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Gene/Protein Information For COL1A1 (Source: Uniprot.org, NCBI)

Alternative Names

Alpha-1 type I collagen; COL1A1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Collagen1; Collagen-1; OI4; pro-alpha-1 collagen type 1 COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain

For more info on COL1A1, check out the COL1A1 Infographic

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In this infographic, you will see the following information for COL1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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